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Design and use of group-specific primers and probes for real-time quantitative PCR

Juntaek LIM, Seung Gu SHIN, Seungyong LEE, Seokhwan HWANG

Frontiers of Environmental Science & Engineering 2011, Volume 5, Issue 1,   Pages 28-39 doi: 10.1007/s11783-011-0302-x

Abstract: Real-time quantitative polymerase chain reaction (qPCR) has gained popularity as a technique to detectAlthough qPCR is a very useful technique for nucleic acid quantification, accurately quantifying theMany aspects of conducting qPCR assays have become increasingly routine and automated; however, one ofWe demonstrate the effectiveness of these guidelines by reviewing the use of qPCR to study anaerobicprocesses and biologic nutrient removal processes. qPCR assays using group-specific primers and probes

Keywords： absolute quantification     design guideline     primer     probe     real-time quantitative polymerase chain reaction(qPCR)    

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Using pyrosequencing and quantitative PCR to analyze microbial communities

Husen ZHANG

Frontiers of Environmental Science & Engineering 2011, Volume 5, Issue 1,   Pages 21-27 doi: 10.1007/s11783-011-0303-9

Abstract: In parallel, quantitative real-time polymerase chain reaction (QPCR) allows quantitative monitoring ofspecific community members over time, space, or different environmental conditions.The best procedures and chemistries for QPCR amplifications are also described and advantages of applyingThree examples in which pyrosequencing and QPCR were used together to define and quantify members of

Keywords： polymerase chain reaction (PCR)     microbial communities     pyrosequencing     gut     microbial fuel cell     sludge    

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hydrophobic environment triggering reactive fluorescence probe to real-time monitor mitochondrial DNA

Frontiers of Chemical Science and Engineering 2022, Volume 16, Issue 1,   Pages 92-102 doi: 10.1007/s11705-021-2063-9

Abstract: i>]imidazole, and malononitrile was introduced as a core into a molecule to initiate the hydrolysis reactioncause additional damages to mitochondrial DNA, it is a potentially effective detection tool for the real-time

Keywords： hydrolysis reaction     mitochondrial DNA damage     in situ hydrophobic environment trigger     fluorescence    

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Convergence to real-time decision making

James M. TIEN

Frontiers of Engineering Management 2020, Volume 7, Issue 2,   Pages 204-222 doi: 10.1007/s42524-019-0040-5

Abstract: Real-time decision making reflects the convergence of several digital technologies, including those concernedwith the promulgation of artificial intelligence and other advanced technologies that underpin real-timeMore specifically, real-time decision making can be depicted in terms of three converging dimensions:), reacting (i.e., making decisions in real-time), and learning (i.e., employing deep neural networksReal-time includes mobile networks, autonomous vehicles, and artificial general intelligence.

Keywords： real-time decision making     services     goods     ServGoods     Big Data     Internet of Things     artificial intelligence    

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WEIS wheel speed real-time measuring method for VOSM

Mengyao PAN, Guixiong LIU, Xiaobin HONG, Tusheng LIN,

Frontiers of Mechanical Engineering 2010, Volume 5, Issue 3,   Pages 322-327 doi: 10.1007/s11465-010-0022-0

Abstract: problems in traditional wheel speed measuring methods, such as low measurement precision and the lack of real-timepacket to implement wavelet de-noising for the non-stationary acceleration signals, and adopting short-timeexperimental conditions the speed measurement error is −2.05%, and the speed measuring response time

Keywords： wheel embedded     intelligent sensing     wheel speed     monitoring    

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Physics-Informed Deep Learning-Based Real-Time Structural Response Prediction Method

Ying Zhou,Shiqiao Meng,Yujie Lou,Qingzhao Kong,

Engineering doi: 10.1016/j.eng.2023.08.011

Abstract: of structural response prediction, this study proposes a novel physics-informed deep-learning-based real-time

Keywords： Structural seismic response prediction     Physics information informed     Real-time prediction     Earthquake engineering    

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Real-time task processing method based on edge computing for spinning CPS

Shiyong YIN, Jinsong BAO, Jie LI, Jie ZHANG

Frontiers of Mechanical Engineering 2019, Volume 14, Issue 3,   Pages 320-331 doi: 10.1007/s11465-019-0542-1

Abstract: Each manufacturing unit in spinning production produces various real-time tasks, which may affect productionEdge computing nodes, real-time tasks, and edge computing resources are defined.A system model is established, and a real-time task processing method is proposed for the edge computingExperimental results indicate that the proposed real-time task processing method based on edge computingcan effectively solve the delay problem of real-time task processing in spinning cyber-physical systems

Keywords： edge computing     real-time task     scheduling     CPS     spinning    

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Virtual network embedding based on real-time topological attributes

Jian DING,Tao HUANG,Jiang LIU,Yun-jie LIU

Frontiers of Information Technology & Electronic Engineering 2015, Volume 16, Issue 2,   Pages 109-118 doi: 10.1631/FITEE.1400147

Abstract: In this paper, a new embedding algorithm is proposed based on real-time topological attributes.

Keywords： Virtual network embedding (VNE)     Real-time topological attributes     Betweenness centrality     Correlation    

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Integration of VR with BIM to facilitate real-time creation of bill of quantities during the design phase

Jordan DAVIDSON, John FOWLER, Charalampos PANTAZIS, Massimo SANNINO, Jordan WALKER, Moslem SHEIKHKHOSHKAR, Farzad Pour RAHIMIAN

Frontiers of Engineering Management 2020, Volume 7, Issue 3,   Pages 396-403 doi: 10.1007/s42524-019-0039-y

Abstract: attributes the declining use of B/Qs to a multitude of reasons, such as its complexity, the potentially long timeThe proposed method features great potential savings in cost and time and gives the B/Q a newfound importance

Keywords： virtual reality     bill of quantities     BIM     Revit     real-time synchronisation    

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A model reference adaptive control based method for actuator delay estimation in real-time testing

Cheng CHEN, James M. RICLES

Frontiers of Structural and Civil Engineering 2010, Volume 4, Issue 3,   Pages 277-286 doi: 10.1007/s11709-010-0072-8

Abstract: Real-time testing provides a viable experimental technique to evaluate the performance of structuralto apply calculated displacements from an integration algorithm to the experimental structures in a real-timetheory enable researchers to accommodate variable actuator delay and achieve good actuator control for real-timeHowever, these adaptive methods all require time duration for actuator delay adaptation.

Keywords： real-time testing     actuator delay     compensation     adaptive control     MIT rule     discrete transfer function    

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A highly selective fluorescent probe for real-time imaging of UDP-glucuronosyltransferase 1A8 in living

Frontiers of Chemical Science and Engineering 2022, Volume 16, Issue 1,   Pages 103-111 doi: 10.1007/s11705-021-2064-8

Abstract: BDMP was successfully used to image endogenous UGT1A8 in human cancer cell lines (LoVo and HCT15) in realtime.

Keywords： UDP-glucuronosyltransferase 1A8     fluorescent probe     subtype selectivity     fluorescence imaging    

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Interaction and independence on methane oxidation of landfill cover soil among three impact factors: water, oxygen and ammonium

Pinjing HE, Na YANG, Wenjuan FANG, Fan Lü, Liming SHAO

Frontiers of Environmental Science & Engineering 2011, Volume 5, Issue 2,   Pages 175-185 doi: 10.1007/s11783-011-0320-8

Abstract: To understand the influence patterns and interactions of three important environmental factors, i.e. soil water content, oxygen concentration, and ammonium addition, on methane oxidation, the soils from landfill cover layers were incubated under full factorial parameter settings. In addition to the methane oxidation rate, the quantities and community structures of methanotrophs were analyzed to determine the methane oxidation capacity of the soils. Canonical correspondence analysis was utilized to distinguish the important impact factors. Water content was found to be the most important factor influencing the methane oxidation rate and Type II methanotrophs, and the optimum value was 15% (w/w), which induced methane oxidation rates 10- and 6- times greater than those observed at 5% (w/w) and 20% (w/w), respectively. Ambient oxygen conditions were more suitable for methane oxidation than 3% oxygen. The addition of of ammonium induced different effects on methane oxidation capacity when conducted at low or high water content. With regard to the methanotrophs, Type II was sensitive to the changes of water content, while Type I was influenced by oxygen content. Furthermore, the methanotrophic acidophile, , was detected in soils with a pH of 4.9, which extended their known living environments.

Keywords： quantitative polymerase chain reaction (PCR)     denaturing gradient gel electrophoresis (DGGE)     principal    

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UsingKinect for real-time emotion recognition via facial expressions

Qi-rong MAO,Xin-yu PAN,Yong-zhao ZHAN,Xiang-jun SHEN

Frontiers of Information Technology & Electronic Engineering 2015, Volume 16, Issue 4,   Pages 272-282 doi: 10.1631/FITEE.1400209

Abstract: In this paper, we propose a real-time emotion recognition approach based on both 2D and 3D facial expressionimproved emotional profiles (IEPs) and maximum confidence is proposed to recognize emotions with these real-timeExperiments on both an emotion dataset and a real-time video show the superior performance of our method

Keywords： Kinect     Emotion recognition     Facial expression     Real-time classification     Fusion algorithm     Support vector    

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Development of a BIM-based holonic system for real-time monitoring of building operational efficiency

Alessandro CARBONARI, Leonardo MESSI, Berardo NATICCHIA, Massimo VACCARINI, Massimiliano PIRANI

Frontiers of Engineering Management 2020, Volume 7, Issue 1,   Pages 89-103 doi: 10.1007/s42524-019-0037-0

Abstract: This approach indicates that a method for automatic real-time diagnosis is tested in a case study consisting

Keywords： BIM     building management system     cyber-physical system     facility management     holonic system    

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Real-time Forecast of Irrigation

Mao Zhi,Li Yuanhua,Li Huichang

Strategic Study of CAE 2002, Volume 4, Issue 5,   Pages 24-33

Abstract:

The real-time forecast of irrigation is one of the essential part of the work for compiling a dynamicThe real-time forecast of water requirements of crops is the essential and difficult part in the real-timeThey can not used for the real-time forecast.Based on the results of the real-time forecast of crop water requirement and precipitation, the real-timeIn this paper, the precision of the real-time forecast and its effects on increasing yield and saving

Keywords： irrigation     real-time forecast     evapotranspiration     irrigation schedule    

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Title Author Date Type Operation

Design and use of group-specific primers and probes for real-time quantitative PCR

Juntaek LIM, Seung Gu SHIN, Seungyong LEE, Seokhwan HWANG

Journal Article

Using pyrosequencing and quantitative PCR to analyze microbial communities

Husen ZHANG

Journal Article

hydrophobic environment triggering reactive fluorescence probe to real-time monitor mitochondrial DNA

Journal Article

Convergence to real-time decision making

James M. TIEN

Journal Article

WEIS wheel speed real-time measuring method for VOSM

Mengyao PAN, Guixiong LIU, Xiaobin HONG, Tusheng LIN,

Journal Article

Physics-Informed Deep Learning-Based Real-Time Structural Response Prediction Method

Ying Zhou,Shiqiao Meng,Yujie Lou,Qingzhao Kong,

Journal Article

Real-time task processing method based on edge computing for spinning CPS

Shiyong YIN, Jinsong BAO, Jie LI, Jie ZHANG

Journal Article

Virtual network embedding based on real-time topological attributes

Jian DING,Tao HUANG,Jiang LIU,Yun-jie LIU

Journal Article

Integration of VR with BIM to facilitate real-time creation of bill of quantities during the design phase

Jordan DAVIDSON, John FOWLER, Charalampos PANTAZIS, Massimo SANNINO, Jordan WALKER, Moslem SHEIKHKHOSHKAR, Farzad Pour RAHIMIAN

Journal Article

A model reference adaptive control based method for actuator delay estimation in real-time testing

Cheng CHEN, James M. RICLES

Journal Article

A highly selective fluorescent probe for real-time imaging of UDP-glucuronosyltransferase 1A8 in living

Journal Article

Interaction and independence on methane oxidation of landfill cover soil among three impact factors: water, oxygen and ammonium

Pinjing HE, Na YANG, Wenjuan FANG, Fan Lü, Liming SHAO

Journal Article

UsingKinect for real-time emotion recognition via facial expressions

Qi-rong MAO,Xin-yu PAN,Yong-zhao ZHAN,Xiang-jun SHEN

Journal Article

Development of a BIM-based holonic system for real-time monitoring of building operational efficiency

Alessandro CARBONARI, Leonardo MESSI, Berardo NATICCHIA, Massimo VACCARINI, Massimiliano PIRANI

Journal Article

Real-time Forecast of Irrigation

Mao Zhi,Li Yuanhua,Li Huichang

Journal Article